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The Queen's Nest is a Zerg structure composed of a festering mound of living organisms that live off Creep and provide the Queen with the abilities of Spawn Broodling and Ensnare as well as Gamete Meiosis. Nutured by the Queen herself, these "children" in turn willingly sacrifice themselves to serve the Overmind as required. Once the Queen's Nest is present, the Lair can continue on its evolutionary path to become a full Hive.
References
Underwood, Peter, Bill Roper, Chris Metzen and Jeffrey Vaughn. StarCraft (Manual). Irvine, Calif.: Blizzard Entertainment, 1998